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KMID : 0545120120220111575
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 11 p.1575 ~ p.1579
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Quantitative Real-Time PCR Assay for Detection of Paenibacillus polymyxa Using Membrane-Fusion Protein-Based Primers
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Cho Min-Seok
Park Dong-Suk
Lee Jung-Won
Chi Hee-Youn
Sohn Soo-In
Jeon Bong-Kyun
Ma Jong-Beom
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Abstract
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Paenibacillus polymyxa is known to be a plant-growthpromoting rhizobacterium. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection and quantitation of P. polymyxa using a primer pair based on the sequence of a membranefusion protein for the amplification of a 268 bp DNA fragment. This study reports that the qPCR-based method is applicable for the rapid and sensitive detection of P. polymyxa and can be used as an alternative method for agricultural soil monitoring.
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KEYWORD
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Paenibacillus polymyxa, detection, quantitation, membrane-fusion protein
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